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KMID : 0613820090190101463
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Journal of Life Science
2009 Volume.19 No. 10 p.1463 ~ p.1467
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Amount of Telomeric DNA on Lymphocytes in Senescence Mouse by Quantitative Fluorescence in situ Hybridization
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Lee Mi-Rang
Do Kyung-Tak
Han Jyung-Ju
Moon So-Hyun
Kang Han-Suk
Kim Seon-Ku
Shin Taek-Soon
Lee Hong-Gu
Hwang Dae-Yon
Kim Yong-Gyun
Sohn Sea-Hwan
Choi Na-Eun
Kim Byung-Woo
Cho Byung-Wook
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Abstract
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Telomeres, comprised of tandem repeats of TTAGGG sequences, are special nucleoprotein structures that protect and stabilize chromosome ends. These structures form the crux of the telomere concept of aging, senescence and genomic instability. The classic terminal restriction fragment (TRF) analysis to quantify the amount of telomeric DNA is disadvantageous in species containing ultra long telomeres like in mice (100Kb). In this study, we used a more sensitive quantitative fluorescence in situ hybridization (Q FISH) technique to quantify telomeric DNA, and used it as a biological aging marker in mice. 12 litters each of Senescence-Resistant (SAMR1) and ?Prone (SAMP1) known as senescence accelerated mouse strains were purchased from Central Lab, Animal Inc. We quantified the amount of telomeric DNA using telomere specific DNA probes on the two strains of male mice at 8 weeks, 18 weeks and 26 weeks of age. The amount of telomeric DNA correlated with aging and age associated changes in body and organ weight between SAMR1 and SAMP1 strains of mice. These data suggest the usefulness of the amount of telomeric DNA as a biological aging marker in human aging studies.
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KEYWORD
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Chromosome, telomere length, Q-FISH, aging, SAM mouse
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